Morphological and molecular variability in cotton (Gossypium hirsutum L.) genotypes from three geographical origins
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Abstract
Cotton (Gossypium hirsutum L.) has lost variability due to its domestication. For this reason, it is necessary to obtain the molecular and phenotypic characterization of new breeding materials for this species. The objective of this work was to characterize the genetic and phenotypic variability of 18 accessions from Argentina, the United States, and China. The materials belong to the INTA (Argentina’s Agricultural Technology Institute) genebank and are used for breeding programs. Sixteen pairs of microsatellite oligonucleotides were used for the study. For the phenotypic characterization, variables associated with mapping were used in two cropping seasons (2015-2016 and 2016-2017) in the INTA experimental field in Sáenz Peña, province of Chaco, Argentina. The genetic results showed medium/high values of polymorphism (62.22%), and the dendrogram showed two groups: one made up of Stoneville 474 (United States) and eight genotypes from China, and a second group made up of Deltapine 16 (United States), the genotypes from Argentina, and one genotype from China. The phenotypic results obtained through Principal Components Analysis showed significant differences between cropping seasons, while no significant differences were detected between geographical origins. The morphological characters of accessions from the three different geographical origins were similar. However, genetic differentiation was found between the microsatellites analyzed, with accessions BGSP 756, BGSP 767 and BGSP 783 from China exhibiting higher genetic distances than materials from both Argentina and the United States. Our results support the introduction of cotton germplasm from China for INTA's breeding program.
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References
ABDALLA, A.; REDDY, O.; EL-ZIK, K. Y PEPPER, A. (2001). Genetic diversity and relationships of diploid and tetraploid cottons revealed using AFLP. Theor Appl Genet. 102:222–229.
BALZARINI, M. Y DI RIENZO, J. Info-Gen versión (2016). FCA, Universidad Nacional de Córdoba, Argentina. URL http://www.info-gen.com.ar
BALZARINI, M.; BRUNO, C.; PEÑA, A.; TEICH, I. Y DI RIENZO, J. (2010). Estadística en Biotecnología en Info-gen. Encuentro grupo Editor. Córdoba, Argentina.
BOTSTEIN, D.; WHITE, R.; SKOLNICK, M. Y DAVIS, R. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American journal of human genetics, 32(3), 314–331.
BRUBAKER, C.; PATERSON, A. Y WENDEL, J. (1999). Comparative genetic mapping of allotetraploid cotton and its diploid progenitors. Genome 42: 184–203.
CHEN, G. Y DU, X. (2006). Genetic Diversity of Source Germplasm of Upland Cotton in China as Determined by SSR Marker Analysis Elsevier. Issue 8., 33 (8):733–745.
DJABOUTOU, M.; SINHA, M.; HOUEDJISSIN, S.; CACAI, G. Y AHANHANZO, C. (2017). Variability and Heritability of Morphological Traits in Collection of Cotton Genotypes (Gossypium hirsutum L.) and Their Potential Use for the Selection. European Scientific Journal, ESJ, 13(3), 385. https://doi.org/10.19044/esj.2017.v13n3p385
ENDRIZZI, J.; TURCOTTE, E. Y KOHEL, R. (1985). Genetics, cytology, and evolution of Gossypium. E.W. Caspari, G.S. John (Eds.), Advances in Genetics, Academic Press, 271-375p.
ETCHART, J. (2009). Detección de polimorfismos moleculares en materiales de Algodón (Gossypium hirsutum L.) fenotípicamente contrastante para la enfermedad azul y su mapeo en una población segregante. [Tésis Magister] Universidad Nacional de Rosario, Argentina, 122 p. https://fcagr.unr.edu.ar/?page_id=142
FANG, D.; HINZE, L.; PERCY, R.; LI, P.; DENG, D. Y THYSSEN, G. (2013). A microsatellite based genome wide analysis of genetic diversity and linkage disequilibrium in Upland cotton (Gossypium hirsutum L.) cultivars from major cotton-growing countries. Euphytica. 191:391–401.
GARRIS, A.; TAI, T.; COBURN, J.; KRESOVICH, S. Y MCCOUCH S. (2005). Genetic structure and diversity in Oryza sativa L. Genetics. 169:1631–1638. [PMC free article] [PubMed].
GENSUS GENETICA SUSTENTABLE: https://gensus.com.ar/productos/.
GIRBAL-BLACHA, N. (2012). La industria invisible: entre las finanzas y la política, empresas de cultura popular en la Argentina peronista: 1946-1955. H-industri@ (B. Aires) Vol. 06 Nro. 11
GÓMEZ, G. (2007). Estimación de la diversidad genética mediante marcadores Microsatélites en entradas de Algodón (Gossypium hirsutum L.) del Banco de germoplasma del INTA. [Tesis Magister] Universidad Nacional de Rosario, Argentina, 80 p.
IRCT, 1946. Institut de recherches du coton et des textiles exotiques. Paris, Francia.
IQBAL, Z.; LATEEF, M.; JABBAR, A.; MUHAMMAD, G. Y KHAN, M. (2005). Anthelmintic activity of Calotropis procera (Ait.) F. flowers in sheep. J. Ethnopharm., 102 (2): 256-261
JENKINS, H. (2003). Transmedia storytelling: Moving characters from books to films to video games can make them stronger and more compelling. MIT Technology.Review.
http://www.technologyreview.com/news/401760/transmedia-storytelling/
LAM, H.; XU, X.; LIU, X.; CHEN, W.; YANG, G.; WONG, F.; LI, M.; HE, W.; QIN, N. Y WANG, B. (2010). Resequencing of 31 wild and cultivated soybean genomes identifies patterns of genetic diversity and selection. Nat Genet. 42:1053–1059. [PubMed].
LI, Y.; LI, W.; ZHANG, C.; YANG, L.; CHANG, R.; GAUT, B. AND QIU LJ. 2010. Genetic diversity in domesticated soybean (Glycine max) and its wild progenitor (Glycine soja) for simple sequence repeat and single-nucleotide polymorphism loci. New Phytol. 188:242–253. [PubMed].
LIU, A. Y BURKE, J. (2006). Patterns of nucleotide diversity in wild and cultivated sunflower. Genetics. 173:321–330. [PMC free article] [PubMed]
LORI, L.; HINZE, E.; GAZAVE, M.; GORE, A.; FANG, D.; SCHEFFLER, B.; YU, J.; JONES, D.; FRELICHOWSKI, J. Y PERCY, R. (2016). Diversidad genética de las dos especies comerciales de algodón tetraploide en Gossypium Diversity Reference Set, Journal of Heredity, volumen 107, número 3, mayo de 2016, páginas 274–286, https://doi.org/10.1093/jhered/esw004
NILES, G. (1980). Plant breeding and improvement of the cotton plant. SAGE Journals 10 (4): 152-158
PEREIRA, G.; SOUSA, R.; HOFFMANN, L.; SILVA, E. Y BARROSO, P. (2012). Selective fertilization in interspecific crosses of allotetraploid species of Gossypium. Botany, v.90, p.159‑166, 2012. DOI: 10.1139/b11‑094
Programa de Asistencia para el Mejoramiento de la Calidad de la Fibra de Algodón (PROCALGODON) (2008). Ex Secretaria de Agricultura, Ganadería, Pesca y Alimentos.
R, DEVELOPMENT CORE TEAM. (2011). R: a language and environment for statistical computing. R Foundation for Statistical Computing. http://www.R-project.org/ verificado: abril de 2011
RAI R, et al. (2013). Gln3 mutations dissociate responses to nitrogen limitation (nitrogen catabolite repression) and rapamycin inhibition of TorC1. J Biol Chem 288(4):2789-804
ROYO, O.; POISSON, J.; BONACIC, I.; MONTENEGRO, A.; IBALO, S.; MAZZA, S. Y GIMÉNEZ, L. (2006). Plant Breeding and Genetics 20pg. https://inta.gob.ar/documentos/direction-of-cotton-breeding-in-argentina
SADRAS, V. (1995). Compensatory growth in cotton after loss of reproductive organs Field Crops Res., 40:.1-18.
SPOLJARIC, M.; TCACH, M.; ROJAS, J.; TARRAGO, J. Y COINTRY, E. (2018a). Caracterización de genotipos de Gossypium hirsutum L. sobre su tolerancia a estrés hídrico Recibido 09 de febrero de 2017 Publicado online 10 de abril de 2018 http://ria.inta.gob.ar/sites/default/files/trabajosenprensa/spoljaric-castellano-4.pdf
SPOLJARIC, M.; TCACH, M.; ROJAS, J.; TARRAGO, J. Y COINTRY, E. (2018b). Caracterización de genotipos de algodón por su tolerancia a estrés hídrico a través de variables asociadas al uso del agua. Revista RG News 4 (2) - Sociedade Brasileira de Recursos Genéticos. http://recursosgeneticos.org/Recursos/Arquivos/3._Caracterizaci_n_de_genotipos_de_algod_n_por_su_tolerancia_a_estr_s_h_drico_a_trav_s_de_variables_asociadas_al_uso_del_agua.pdf
TYAGI, P.; GORE, M.; BOWMAN, D.; CAMPBELL, B.; UDALL, J. Y KURAPARTHY, V. (2014). Genetic diversity and population structure in the US Upland cotton (Gossypium hirsutum L.). Theor Appl Genet. 127:283–295. [PubMed].
VIGOUROUX, Y.; MITCHELL, S.; MATSUOKA, Y.; HAMBLIN, M.; KRESOVICH, S.; SMITH, J.; JAQUETH, J.; SMITH, O. Y DOEBLEY, J. (2005). An analysis of genetic diversity across the maize genome using microsatellites. Genetics. 169:1617–1630. [PMC free article] [PubMed].
WANG, J.; AGRAWALA, M. Y COHEN, M. (2007) "Soft scissors: An interactive tool for realtime high quality matting", SIGGRAPH'07.
WENDEL, J.; BRUBAKER, C. Y PERCIVAL, A. (1992). Genetic diversity in Gossypium hirsutum and the origin of upland cotton. Am J Bot. 79:1291–1310.
WENDEL, J., BRUBAKER, C., Y SEELANAN, T. (2010). The origin and evolution of Gossypium. In JM. Stewart, D. Oosterhuis, J. J. Heithholt, & J. R. Mauney (Eds.), Physiology of cotton (pp. 1–18). Dordrecht, The Netherlands: Springer.
YU J., JUNG S., CHENG CH., LEE T., ZHENG P., BUBLE K., CRABB J., HUMANN J., HOUGH H., JONES D., CAMPBELL J., UDALL J., MAIN D. (2021). CottonGen: la base de datos comunitaria para la genómica del algodón, genética, y la investigación de mejoramiento. plantas 2021; 10(12):2805. https://www.cottongen.org/